Anti-LC3 (Human) mAb
Cat# M152-3
Size : 200μg
Marca : MBL International
M152-3
Antibody
200 µg/100 µL
This LC3 antibody is validated for multiple applications (ICC, IH, IP, FCM, Immuno-EM) and has numerous citations. This is a monoclonal antibody targeting LC3 for FCM, ICC, IP.
More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.
Target | LC3 |
---|---|
Product Type | Antibody |
Application | FCM, ICC, IHC, Immuno-EM, IP |
Clonality | Monoclonal |
Conjugate | Unlabeled |
Isotype | IgG1 ĸ |
Immunogen | Recombinant human LC3 (MAP1LC3B: 1-120 a.a.) |
Host Species | Mouse |
Species Reactivity | Human, Hamster, Mouse, Rat |
Formulation | 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
Research Area | Autophagy |
Description/Background | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3. |
Storage Temperature | -20°C |
Protocols | FCM, ICC, IHC, Immuno-EM, IP |
Clone Number | 4E12 |
Concentration | 2 mg/mL |
Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
M152-3
Antibody
200 µg/100 µL