Anti-MALTOSE PHOSPHORYLASE (E.coli) (GOAT) Antibody Biotin Conjugate

Cat# 200-106-230-0100

Size : 100ug

Marca : Rockland Immunochemicals

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Specifications for Maltose Phosphorylase Antibody Biotin Conjugated

Product Details

Anti-Maltose Phosphorylase (E. coli) (GOAT) Antibody Biotin Conjugated (BULK ORDER) - 200-106-230
goat anti-Maltose Phosphorylase Antibody biotin Conjugation, Biotin Conjugated goat anti-Maltose Phosphorylase Antibody, mapA antibody, mpA antibody
Goat
Biotin
Polyclonal
IgG

Target Details

LAF  - View All LAF Products
Bacteria
Native Protein
Maltose Phosphorylase [E.coli]
Anti-Maltose Phosphorylase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase [E.coli].  Cross reactivity against Maltose Phosphorylase from other sources is unknown.
B2GEX2 - UniProtKB
YP_001842841.1 - NCBI Protein
6233598 - Gene ID

Application Details

Anti-Maltose Phosphorylase antibody has been assayed against 1.0 µg of Maltose Phosphorylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

Background

Anti-Maltose Phosphorylase recognizes the protein maltose phosphorylase which belongs to the glycosyltransferases family. Maltose phosphorylase catalyzes the reaction in which maltose and inorganic phosphate are converted to D-glucose and beta-D-glucose 1-phosphate.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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