Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Anti-GFAP-R416WT Antibody FL550 Conjugate

Stampa

Cat# 75-279-FL550

Size : 200uL

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : Antibodies Incorporated

Contatta il distributore locale :

Telefono : +1 850 650 7790

Our Anti-GFAP-R416WT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206B/9. It is KO validated, detects human, mouse, and rat GFAP-R416WT, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Mouse

Available Conjugates

Unconjugated

490

594

650

Data Sheet

KO Validated

SKU: 75-279-FL550

Product Details

Target

GFAP-R416WT

Target Description

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers .

Format

Purified by Protein A chromatography

Concentration

0.5 mg/mL

Clonality

Monoclonal

Clone

N206B/9

Isotype

IgG1

Applications

ICC, IHC

Host Species

Mouse

Gene Name

GFAP

Molecular Weight

50 kDa

Antigen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2939993

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL550 Ex: 550 nm, Em: 575 nm

Specificity

Does not cross-react with GFAP-R416W or other proteins (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Glial fibrillary acidic protein (GFAP)

UniProt Details

UniProt (Human): P14136
UniProt (Immunogen Species): P14136