Anti-Phospho-CTTN-Tyr466 antibody (441-490 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CTTN
Gene ID:	2017
Uniprot ID:	SRC8_HUMAN
Immunogen Region:	441-490 aa
Specificity:	Phospho-Cortactin (Y466) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y466.
Immunogen:	The antiserum was produced against synthesized peptide derived from mouse Cortactin around the phosphorylation site of Tyr466 at the amino acid range 441-490

Function	Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Protein Name	Src Substrate Cortactin Amplaxin Oncogene Ems1
Database Links	Reactome: R-HSA-5627123 Reactome: R-HSA-8856828
Cellular Localisation	Cytoplasm Cytoskeleton Cell Projection Lamellipodium Ruffle Dendrite Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Podosome Cell Junction Focal Adhesion Membrane Clathrin-Coated Pit Dendritic Spine Cell Cortex Endoplasmic Reticulum Colocalizes Transiently With Ptk2/Fak1 At Focal Adhesions Associated With Membrane Ruffles And Lamellipodia In The Presence Of Cttnbp2nl Colocalizes With Stress Fibers In The Presence Of Cttnbp2 Localizes At The Cell Cortex In Response To Neuronal Activation By Glutamate Redistributes From Dendritic Spines To The Dendritic Shaft Colocalizes With Dnm2 At The Basis Of Filopodia In Hippocampus Neuron Growth Zones
Alternative Antibody Names	Anti-Src Substrate Cortactin antibody Anti-Amplaxin antibody Anti-Oncogene Ems1 antibody Anti-CTTN antibody Anti-EMS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance