General Info
| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-LIM domain kinase 2-Ser283 (249-298 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | LIMK2 |
| Gene ID: | 3985 |
| Uniprot ID: | LIMK2_HUMAN |
| Immunogen Region: | 249-298 aa |
| Specificity: | Phospho-LIMK-2 (S283) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at S283. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human LIMK2 around the phosphorylation site of Ser283 at the amino acid range 249-298 |
Description
| Function | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways.phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. |
| Protein Name | Lim Domain Kinase 2Limk-2 |
| Database Links | Reactome: R-HSA-3928662Reactome: R-HSA-416572Reactome: R-HSA-5627117 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleMicrotubule Organizing CenterCentrosomeIsoform Limk2a: CytoplasmNucleusIsoform Limk2b: CytoplasmPerinuclear RegionMainly Present In The Cytoplasm And Is Scarcely Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Lim Domain Kinase 2 antibodyAnti-Limk-2 antibodyAnti-LIMK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance