ASR3543 | Anti-Rat IgG F(ab')2 Secondary Antibody Clinisciences

Product Information
Description	Anti-RAT IgG F(ab')2 (RABBIT) Antibody
Host	Rabbit
Conjugate	Unconjugated
Target Species	Rat
Clonality	Polyclonal
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC, E
Application Note	ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunohistochemistry 1:1,000-1:5,000
Physical State	Lyophilized
Host Isotype	IgG
Target Isotype	IgG F(ab')2
Buffer	0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen	Rat IgG F(ab')2 fragment
Reconstitution Volume	5.0 mL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	None
Preservative	None

Additional Information
Shipping Condition	Ambient
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c).
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Anti-Rat IgG F(ab')2 Secondary Antibody