Size : 100ml
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Introduction Procedures to eliminate background comprise three main steps:
AURION BSA-c™ is a particularly effective reagent for this purpose.
AURION BSA-c™ is a buffer additive that helps prevent immunodetection reagents (i.e. primary antibodies and secondary reagents) from binding nonspecifically to charged moieties within the specimen. Thus, it suppresses background competitively with little or no effect on the specific reaction. Its successful application is not limited to immunogold detections but it is equally efficient in fluorescent and enzyme-based detection systems. AURION BSA-c™ concentrations as low as 0.01-0.1% inhibit binding of%).
The surface properties of the specimen can be simplified by division into four compartments: – negatively charged (polyanions, proteins, especially after aldehyde fixation, lipids); 0; competes with the negatively charged antibodies and gold reagents for non-specific binding to the positively charged specimen compounds, thus reducing background to the greatest possible extent without interfering with antigen detection.
Product Description AURION BSA-c™ concentrated solution contains acetylated bovine serum albumin as the functional constituent. By acetylation of amines on basic amino acids these groups are no longer as easily protonated and the isoelectric point of such molecules is lowered and hydrophobicity is increased. BSA-c™ is a 10% solution of acetylated BSA at slightly alkaline pH with Kathon CG as preservative. The bovine serum albumin that Aurion uses to prepare BSA-c™ is obtained from healthy livestock. The charge dependent background inhibition capacity of the BSA-c™ in each lot is determined using a dot-spot test system with polycationic compounds. Two package volumes are available which yield 1.5 – 3 and 5 -10 litres of incubation buffer respectively
Application Instructions Detailed information is provided in the package inserts.
Recommended Incubation Solution:
PBS, (10 mM Phosphate buffer, 150 mM NaCl) 0.1-0.2 % AURION BSA-c™ 15 mM NaN3 pH 7.4
After addition of the BSA-c™ concentrate the pH should be checked and adjusted to 7.4 if necessary. This Incubation Solution is used throughout the whole set of reactions and washing steps (i.e. from immediately after the blocking step, through the primary antibody incubations, in-between washing steps, secondary incubation and the final washing steps).
Note on background prevention: A special AURION Newsletter on background is available in the Sharing our Knowledge section of this website.
Storage AURION BSA-c™ concentrate has a guaranteed shelf life of 18 months from the date of quality control analysis. The products should be stored at 4-8" class="widget widget_black_studio_tinymce">
Concentrated buffer additive Unparalleled background suppression For immunohisto and cytochemistry For immunogold detection systems For enzyme based detection systems For fluorescent detection systems For EM grids and LM specimens For bio assays
AURION BSA-c 2 - 100 ml concentrate (10%) 9 - 30%)
Aurion BSA-c,CgkJdmFyIHJlY2FwdGNoYV93aWRnZXRzPXt9OwoJCWZ1bmN0aW9uIHdwX3JlY2FwdGNoYUxvYWRDYWxsYmFjaygpewoJCQl0cnkgewoJCQkJZ3JlY2FwdGNoYTsKCQkJfSBjYXRjaChlcnIpewoJCQkJcmV0dXJuOwoJCQl9CgkJCXZhciBlID0gZG9jdW1lbnQucXVlcnlTZWxlY3RvckFsbCA/IGRvY3VtZW50LnF1ZXJ5U2VsZWN0b3JBbGwoJy5nLXJlY2FwdGNoYTpub3QoLndwY2Y3LWZvcm0tY29udHJvbCknKSA6IGRvY3VtZW50LmdldEVsZW1lbnRzQnlDbGFzc05hbWUoJ2ctcmVjYXB0Y2hhJyksCgkJCQlmb3JtX3N1Ym1pdHM7CgoJCQlmb3IgKHZhciBpPTA7aTxlLmxlbmd0aDtpKyspIHsKCQkJCShmdW5jdGlvbihlbCl7CgkJCQkJdmFyIHdpZDsKCQkJCQkvLyBjaGVjayBpZiBjYXB0Y2hhIGVsZW1lbnQgaXMgdW5yZW5kZXJlZAoJCQkJCWlmICggISBlbC5jaGlsZE5vZGVzLmxlbmd0aCkgewoJCQkJCQl3aWQgPSBncmVjYXB0Y2hhLnJlbmRlcihlbCx7CgkJCQkJCQknc2l0ZWtleSc6JzZMZVRvNFlVQUFBQUFJNTlkNkVzMmphajJmdU53cXNGekF3TUdHX3knLAoJCQkJCQkJJ3RoZW1lJzplbC5nZXRBdHRyaWJ1dGUoJ2RhdGEtdGhlbWUnKSB8fCAnbGlnaHQnCgkJCQkJCX0pOwoJCQkJCQllbC5zZXRBdHRyaWJ1dGUoJ2RhdGEtd2lkZ2V0LWlkJyx3aWQpOwoJCQkJCX0gZWxzZSB7CgkJCQkJCXdpZCA9IGVsLmdldEF0dHJpYnV0ZSgnZGF0YS13aWRnZXQtaWQnKTsKCQkJCQkJZ3JlY2FwdGNoYS5yZXNldCh3aWQpOwoJCQkJCX0KCQkJCX0pKGVbaV0pOwoJCQl9CgkJfQoKCQkvLyBpZiBqcXVlcnkgcHJlc2VudCByZS1yZW5kZXIganF1ZXJ5L2FqYXggbG9hZGVkIGNhcHRjaGEgZWxlbWVudHMKCQlpZiAoIHR5cGVvZiBqUXVlcnkgIT09ICd1bmRlZmluZWQnICkKCQkJalF1ZXJ5KGRvY3VtZW50KS5hamF4Q29tcGxldGUoIGZ1bmN0aW9uKGV2dCx4aHIsc2V0KXsKCQkJCWlmKCB4aHIucmVzcG9uc2VUZXh0ICYmIHhoci5yZXNwb25zZVRleHQuaW5kZXhPZignNkxlVG80WVVBQUFBQUk1OWQ2RXMyamFqMmZ1Tndxc0Z6QXdNR0dfeScpICE9PSAtMSkKCQkJCQl3cF9yZWNhcHRjaGFMb2FkQ2FsbGJhY2soKTsKCQkJfSApOwoKCQk=">
Telefono : +1 850 650 7790
Telefono : +1 850 650 7790
