Disease:

Ductal carcinoma

Origin:

The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes.

Species:

Homo sapiens, human

Tissue:

mammary gland; breast

Morphology:

Epithelial. The established population is polymorphic with epithelial-like components and multinucleated giant cells. A mucin-like material was secreted into the medium.

Properties:

Adherent

Cytogenic data:

modal number = 78; range = 73 to 80. The cell line is aneuploid human female, with chromosome counts in the hypertriploid range. The X chromosomes are all abnormal. Normal chromosomes N10 and N13 are clearly under-represented, with chromosomes N2, N12, and N17 also tending to be. Chromosome N8 is over-represented with respect to the copy number of other normal chromosomes, and chromosomes N5 and N18 also usually have more copies than most chromosomes. Four marker chromosomes are found: iso(13q), 10q+, del(X)(q22:), del(11)(p11:).

Patient:

Female, Caucasian, 72 years of age.

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS + Insulin (0.023 IU/mL)

Subculture:

Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 to 1:6, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO