CA125 ELISA Kit (Mouse)

Cat# OKCD04366

Size : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for CA125 ELISA Kit (Mouse) (OKCD04366)
Product Info
Predicted Species ReactivityMouse
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-101%80-89%95-103%98-105%
EDTA Plasma (n=5)97-105%92-101%98-105%78-104%
Heparin Plasma (n=5)91-103%80-101%91-98%80-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 125 (CA125). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbohydrate Antigen 125 (CA125). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 125 (CA125), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbohydrate Antigen 125 (CA125) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)80-9186
EDTA Plasma (n=5)92-105101
Heparin Plasma (n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbohydrate Antigen 125 (CA125) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbohydrate Antigen 125 (CA125) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ca125 Microplate96 Wells (12 x 8 Well strips)
Ca125 Lyophilized Standard2
100X Ca125 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.5 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCA125
Protein NameCarbohydrate Antigen 125
Uniprot IDQ9D1H1

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