CCL4 ELISA Kit (Dog)

Cat# OKEH07285

Size : 96T

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CCL4 ELISA Kit (Dog) (OKEH07285) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL4 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.0% (n = 20)
Mean Inter-assay CV%: <=8.0% (n = 20)
ELISA Kit Component
ComponentAmount
CCL4 Microplate96 Wells (12 x 8 Well strips)
CCL4 Lyophilized Standard2
100X Biotinylated CCL4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Monokine with inflammatory and chemokinetic properties.
::Subcellular Location: Secreted
Reconstitution and StorageStore as indicated in product manual.
Sensitivity7.8pg/mL
SpecificityNatural and recombinant Dog C-C motif chemokine 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCL4
Gene Full Namechemokine (C-C motif) ligand 4
Alias SymbolsCC chemokine ligand 4;C-C motif chemokine 4;SCYA4;small-inducible cytokine A4.
NCBI Gene Id448786
Protein NameQ68AZ0
Description of TargetC-C motif chemokine 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q68AZ0
Protein Accession #NP_001005250.1
Nucleotide Accession #NM_001005250.1