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CD5 (APC)

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Cat# 227428-APC-100ul

Size : 100ul

Marca : US Biological

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227428-APC CD5 (APC)

Clone Type
Polyclonal
Host
mouse
Source
equine
Isotype
IgG1
Grade
Affinity Purified
Applications
IHC IP
Crossreactivity
Eq
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes the equine CD5 antigen, a single-pass type I membrane protein with a molecular weight of ~69kD. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.||Applications: |Suitable for use in Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunohistochemistry: frozen|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CVS5|Host: mouse|Source: equine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Equine leucocytes.|Specificity: Recognizes equine CD5.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Equine leucocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes equine CD5.
References
1. Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunology 74: 251-257. 2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62: 101-143. 3. Moyo, N.A. et al. (2013) Differentiation and activation of equine monocyte-derived dendritic cells is not correlated with CD206 or CD83 expression. Immunology. Mar 5. [Epub ahead of print]. 4. Mayall, S. et al. (2001) The anti-human CD21 antibody, BU33, identifies equine B cells. J Comp Pathol. 124: 83-7. 5. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells. Vet Immunol Immunopathol. 60: 15-31.