CD8 (APC)

Cat# 214732-APC-100ul

Size : 100ul

Marca : US Biological

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214732-APC CD8 (APC)

Clone Type
Polyclonal
Host
mouse
Source
equine
Isotype
IgG1
Grade
Affinity Purified
Applications
IHC IP
Crossreactivity
Eq
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.||A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007).||Applications: |Suitable for use in Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Hybridoma: |X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CVS8|Host: mouse|Source: equine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Equine PBMC|Specificity: Recognizes equine CD8. Species Crossreactivity: Ass (Equus asinus)||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Equine PBMC
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes equine CD8. Species Crossreactivity: Ass (Equus asinus)
References
1. Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets Immunology 74: 251-257. 2. Jacks, S. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction. Clin Vaccine Immunol. 14: 669-77. 3. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7. 4. Lunn, D.P. et al. (1998) Report of the second equine leucocyte antigen workshop, Squaw Valley, California July 1995. Vet Immunol Immunopathol. 62: 101-143. 5. Merant, C. et al. (2003) Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry. Vet Res. 34: 791-801. 6. Ibrahim, S (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28 Chapter 5 in PhD Thesis Freie Universität Berlin