Product Description

CHAPS
Catalog number: B2010157
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 1 g
pH: na
Supplied as: Ready-to-use
Appearance: Solution
Applications: multipurpose non-denaturing zwitterionic detergent. CHAPS has many applications in protein solubilization (especially membrane proteins). It has been used in molecular biology, biochemistry and clinical chemistry.
Storage: Room temperature
Keywords: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Tao H, Liu W, Simmons BN, Harris HK, Cox TC, Massiah MA. Purifying natively
folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS.
Biotechniques. 2010 Jan;48(1):61-4.

2: Kieber M, Ono T, Oliver RC, Nyenhuis SB, Tieleman DP, Columbus L. The
Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS.
Biophys J. 2019 May 7;116(9):1682-1691.

3: Massiah MA, Wright KM, Du H. Obtaining Soluble Folded Proteins from Inclusion
Bodies Using Sarkosyl, Triton X-100, and CHAPS: Application to LB and M9 Minimal
Media. Curr Protoc Protein Sci. 2016 Apr 1;84:6.13.1-6.13.24.

4: Hwang E, Cheong HK, Kim SY, Kwon O, Blain KY, Choe S, Yeo KJ, Jung YW, Jeon
YH, Cheong C. Crystal structure of the EnvZ periplasmic domain with CHAPS. FEBS
Lett. 2017 May;591(10):1419-1428.

5: Rodi PM, Bocco Gianello MD, Corregido MC, Gennaro AM. Comparative study of
the interaction of CHAPS and Triton X-100 with the erythrocyte membrane. Biochim
Biophys Acta. 2014 Mar;1838(3):859-66.

6: Naranjo AN, McNeely PM, Katsaras J, Robinson AS. Impact of purification
conditions and history on A2A adenosine receptor activity: The role of CHAPS and
lipids. Protein Expr Purif. 2016 Aug;124:62-7.

7: Herrera FE, Garay AS, Rodrigues DE. Structural properties of CHAPS micelles,
studied by molecular dynamics simulations. J Phys Chem B. 2014 Apr
10;118(14):3912-21.

8: Qin X, Liu M, Yang D, Zhang X. Concentration-dependent aggregation of CHAP S
investigated by NMR spectroscopy. J Phys Chem B. 2010 Mar 25;114(11):3863-8.

9: Musante L, Saraswat M, Duriez E, Byrne B, Ravidà A, Domon B, Holthofer H.
Biochemical and physical characterisation of urinary nanovesicles following
CHAPS treatment. PLoS One. 2012;7(7):e37279.

10: Qin X, Liu M, Zhang X, Yang D. Proton NMR based investigation of the effects
of temperature and NaCl on micellar properties of CHAPS. J Phys Chem B. 2011 Mar
10;115(9):1991-8.

11: Yao S, Westphal D, Babon JJ, Thompson GV, Robin AY, Adams JM, Colman PM,
Czabotar PE. NMR studies of interactions between Bax and BH3 domain-containing
peptides in the absence and presence of CHAP S. Arch Biochem Biophys. 2014 Mar
1;545:33-43.

12: Naskar B, Ghosh S, Nagadome S, Sugihara G, Moulik SP. Behavior of the
amphiphile CHAP S alone and in combination with the biopolymer inulin in water
and isopropanol-water media. Langmuir. 2011 Aug 2;27(15):9148-59.

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