Choline Oxidase antibody (Biotin)
Cat# orb344283-100ug
Size : 100ug
Marca : Biorbyt
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Telefono : +1 850 650 7790
Choline Oxidase antibody (Biotin)
Catalog Number: orb344283
| Catalog Number | orb344283 |
|---|---|
| Category | Antibodies |
| Description | Choline Oxidase antibody (Biotin) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Choline Oxidase [Alcaligenes species] |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown. |
| Conjugation | Biotin |
| UniProt ID | P16101 |
| NCBI | 116367 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | goat anti-Choline Oxidase Antibody biotin Conjugat |
| Note | For research use only |
| Application notes | Anti-Choline Oxidase has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 ug of Choline Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti Choline Oxidase antibody was used to detect Choline Oxidase. Samples of ~1 and 0.25 ug of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline Oxidase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Biorbyt Goat anti Choline Oxidase. Primary antibody was detected with Biorbyt Dylight 649 conjugated.
Western Blot of Goat anti-Choline Oxidase (Alcaligenes species) Antibody Biotin Conjugated. Lane 1: Choline Oxidase (Alcaligenes species). Load: 50 ng per lane. Primary antibody: Goat anti-Choline Oxidase (Alcaligenes species) Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Choline Oxidase.