Collagen I, rat tail (Rat)
Cat# OPED00033
Size : 20mg
Marca : Aviva Systems Biology
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Telefono : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Collagen I, rat tail (Rat) (OPED00033) (OPED00033) |
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| Predicted Species Reactivity | Rat |
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| Product Format | Liquid 0.02N acetic acid Physical Appearance: Hazy viscous liquid |
| Host | Rat |
| Additional Information | Endotoxin Content: <100 EU/mg purified protein (LAL test) |
| Reconstitution and Storage | Store at 4°C. Keep sterile. Do not freeze. |
| Concentration | Varies by lot. See vial for concentration. |
| Procedure | Firm Gelling Procedure (Note: The ideal concentration for gelling is 1 - 5mg/ml. The end user will need to determine the appropriate concentration for their specific purpose). Ammonium Hydroxide Gas Method Dilute 5mg/ml collagen to the desired concentration using sterile conditions. (Note: Lower concentrations will have reduced rigidity). Add enough collagen to cover the surface. Saturate a cotton ball or piece of filter paper with concentrated ammonium hydroxide and make a closed vapor chamber. Heat to 37C until gel forms. Remove the ammonium hydroxide. Soak the gel surface in PBS for 1 hour. Wash several times PBS. Store the gel in PBS at 4C until use. Equilibrate the gel in the desired media for 30 minutes prior to use. NaOH Method (For 10ml) Sterilely add 1- 7ml of 5mg/ml collagen to a tube depending on desired final concentration. (Note: Lower concentrations will have reduced rigidity). Add 1ml of sterile 10X PBS. Add 1 ml of sterile 1N NaOH. Bring final volume to 10ml with sterile dH2O Cover the desired surface with a layer of the collagen and place at 37C until the gel has solidified. Gel will only form if the pH is >=7.0. Store in PBS at 4C until use. Equilibrate the gel in the desired media for 30 minutes prior to use. |
| Purity | >90% (SDS-PAGE) |
| Biological Activity | Tested in cell proliferation assay -- Increased attachment of cells on collagen coated coverslips. |
| Source | Isolated from rat tail tendons. |
| Application Info | Can be used for preparation of collagen gels, thin layer coating of surfaces (culture plates, slides, coverslips). |
| Gene Symbol | COLLAGEN I |
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| Protein Name | Collagen |
| Description of Target | Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1(I) and one alpha-2(I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment. |
| Protein Accession # | NP_002147.2 |
| Nucleotide Accession # | NM_002156.4 |
| Molecular Weight | 235 kDa, 215 kDa, 130 kDa, 115 kDa |