CRP ELISA Kit (Rabbit)

Cat# OKIA00124

Size : 1x96wellplate

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKIA00124
Product Info
Predicted Species ReactivityRabbit
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration25 Minutes
ELISA Kit PrincipleAviva Systems Biology CRP ELISA Kit (Rabbit) (OKIA00124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for C-reactive protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-C-reactive protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C-reactive protein captured in the well.
ELISA Kit Range6.25 ng/ml - 200 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rabbit CRP Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rabbit CRP Calibrator1
Additional InformationIntended Use: The CRP test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in Rabbit biological samples.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rabbit CRP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RABBIT CRP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rabbit CRP.
7. RABBIT CRP CALIBRATOR
One vial containing a lyophilized Rabbit CRP calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.610 ng/ml
Predicted Homology Based on Immunogen SequenceRabbit
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsPTX1
NCBI Gene Id100009091
Protein NameC-reactive protein
Description of TargetDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Uniprot IDP02742

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