CRTAC1 ELISA Kit (Mouse)

Cat# OKEH01822

Size : 96T

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CRTAC1 ELISA Kit (Mouse) (OKEH01822) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRTAC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRTAC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRTAC1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
CRTAC1 Microplate96 Wells (12 x 8 Well strips)
CRTAC1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CRTAC1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity41.8 pg/mL
SpecificityNatural and recombinant Mouse Cartilage acidic protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCrtac1
Gene Full Namecartilage acidic protein 1
Alias Symbols2810454P21Rik;68 kDa chondrocyte-expressed protein;ASPIC;AW047536;cartilage acidic protein 1;CEP-68;Crtac;Crtac1B;Lo;Lotus;protein CRTAC1-B.
NCBI Gene Id72832
Protein NameQ8R555
Description of TargetCartilage acidic protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q8R555
Protein Accession #NP_660105.3
Nucleotide Accession #NM_145123.4
Protein Size (# AA)646
Molecular Weight70 kDa