Cytochrome c Apoptosis Assay Kit
Cat# K2104-200ug
Size : 200ug
Marca : APExBIO Technology
Contatta il distributore locale :
Telefono : +1 850 650 7790
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Cytochrome c Apoptosis Assay Kit
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Cytochrome c is located between the inner and the outer mitochondrial membranes. Once activated by the apoptosis stimulus, cytochrome c is released from mitochondria into cytosol where it couples to Apaf-1. The cytochrome c/Apaf-1 complex further activates the downstream caspases. The Cytochrome c Releasing Apoptosis Assay Kit gives an efficient way for sensing cytochrome c releasing from mitochondria into cytosol under apoptosis. The kit utilizes a special formulation of reagents to isolate enriched mitochondrial fraction from cytosol. The protocol is simple to perform without unltracentrifugations and toxic chemicals. The cytochrome c antibody provided in the kit is then used to determine the Cytochrome c releasing from mitochondria into cytosol by Western blotting.
| Features | Simple procedure; Takes approx. 3 hours; Fast and convenient; Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required. |
| Shipping | Dry Ice |
| Storage Conditions | Multi |
Datasheet| Kit components | Mitochondria Extraction Buffer, 5X Cytosol Extraction Buffer, DTT (1 M, 500X Protease Inhibitor Cocktail, Anti-Cytochrome c mouse mAb (0.2 mg/mL) |