Deoxyribonuclease I from Bovine Pancreas min. 3000 Kunitz units/mg
Cat# 18535.01
Size : 25MG
Marca : SERVA Electrophoresis
(DNase I, Deoxyribonucleodepolymerase, Deoxyribonucleate 5'-oligo-nucleotide hydrolase, Desoxyribonuclease, Nuklease, Ribonuklease, )
EC 3.1.21.1 • Mr 31 000 • CAS [9003-98-9]
EC 3.1.21.1 • Mr 31 000 • CAS [9003-98-9]
Deoxyribonuclease I, or DNase I
DNase I is an endonuclease that cleaves phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. It preferentially cleaves double-stranded DNA and, in the presence of Mg2+ , single-stranded DNA.
Unit definition: 1 Kunitz unit catalyses an increase in absorbance of 0.001 at 260 nm per minute at 25 °C, pH 5.0, when exposed to highly polymerised calf thymus DNA (1).
Activity: min. 3000 Kunitz units/mg lyophilisate
References:
- Kunitz, M. (1950) J. Gen. Physiol. 33, 349-62
- Laskowski, M. Sr. (1971) The Enzymes IV, 3rd Ed. (Boyer, P.D.ed.) Acad. Press N.Y. 289-311
- Moore, S. (1981) The Enzymes XIV, 3rd Ed. (Boyer, P.D. ed.) Acad. Press N.Y. 281-96
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (5.83, 10.6-10.12, 15.14, 15.16, 15.27-15.31, 13.24-13.25, 13.28-13.29)
EINECS: 232-667-0 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C