Dithiothreitol, as well called DTT or Cleland's reagent, quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE). The reducing thiol agent cleaves disulfide bonds between cysteine residues, thus totally unfolding proteins to allow a separation according to the molecular weight. It may also be used in lysis buffer in protein sample preparations. It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed. Oxidized form of DTT max. 0.5 %. Hygroscopic.

Assay (titr.) MP

min. 99.0 % 40 - 45 °C

References:

1. Cleland, W.W. (1964) Biochemistry 3, 480-2

WARNING

Hazard Statements	H302 - H315 - H319 - H335
Precautions	P264 - P280
Reaction	P301 +P312 - P302 +P352 - P304 +P340 - P305 +P351 +P338

EINECS: 222-468-7 • WGK: 3L • HS: 29309095
Storage Temperature: +2 °C to +8 °C