Donkey normal serum

Cat# AS101563

Size : 2ml

Marca : Agrisera

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Donkey normal serum (2 ml)

Product no: AS10 1563
AS10 1563 | Host: Donkey

For larger volumes, please inquire
    Product Info
    Host: Donkey
    Purity: Serum
    Format: Lyophilized
    Quantity: 2 ml
    Reconstitution: For reconstitution add 2.2 ml of sterile water
    Storage: Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 2.2 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
    Additional Information
    Additional information:

    Concentration: 60.0 mg/ml (Bradford, IgG standards)

    Recommended dilution: 2-5% (v/v) serum in PBS/TBS.  To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.

    For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.

    Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.

    Background
    Background: Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
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Cat#
Descrizione
Cond.
Priced
900.122
 5ml