Visibly dyed microspheres are frequently used in lateral flow tests and "latex" agglutination tests. Our polystyrene microspheres are impregnated with vibrant dyes for optimal visualization. The colorpalette for a representation of our standard colors. Non-functionalized and functionalized versions are available to support adsorption and covalent binding strategies. |
Dyed polystyrene
Our non-functionalized microspheres are suitable for coating with antibody or other large proteins via adsorption. Unless noted otherwise, these visibly dyed microspheres are supplied as a 5% solids suspension (w/v) and are available in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.
TechNote 204, Adsorption To Microspheres
PDS 717, Visibly Dyed Microspheres
Catalog Number | Dye | Nominal Diameter | Specification Range |
---|---|---|---|
DSCR001 | Crimson Red | 0.050µm | 0.040 - 0.060µm |
DSCB002 | Cabo Blue | 0.200µm | 0.190 - 0.210µm |
DSCR002 | Crimson Red | 0.200µm | 0.190 - 0.210µm |
DSSG002 | Shamrock Green | 0.200µm | 0.190 - 0.210µm |
DSCR003 | Crimson Red | 0.300µm | 0.270 - 0.330µm |
DSCR004 | Crimson Red | 0.400µm | 0.370 - 0.430µm |
DSCB005 | Cabo Blue | 0.80µm | 0.770 - 0.830µm |
DSCR005 | Crimson Red | 0.80µm | 0.770 - 0.830µm |
DSSG005 | Shamrock Green | 0.80µm | 0.770 - 0.830µm |
DSCR006 | Crimson Red | 5.0µm | 4.80 - 5.20µm |
References
Henderson, K., & Stewart, J. (2002). Factors influencing the measurement of oestrone sulphate by dipstick particle capture immunoassay. Journal of immunological methods, 270(1), 77-84.(0.3, 0.5, and 0.8µm dyed blue PS)
Henderson, K., & Stewart, J. (2000). A dipstick immunoassay to rapidly measure serum oestrone sulfate concentrations in horses. Reproduction, Fertility and Development, 12(4), 183-189.(0.31µm dyed blue PS)
Campbell, K., Fodey, T., Flint, J., Danks, C., Danaher, M., O'Keeffe, M., ... & Elliott, C. (2007). Development and validation of a lateral flow device for the detection of nicarbazin contamination in poultry feeds. Journal of agricultural and food chemistry, 55(6), 2497-2503.(0.43µm dyed blue PS)
DS03B, DS03G,DS03R,DS03O,DS03K,DS03V, DS03N
DS04B, DS04G,DS04R,DS04O,DS04K,DS04V, DS04N
DS05B, DS05G,DS05R,DS05O,DS05K,DS05V, DS05N
DS06B, DS06G,DS06R,DS06O,DS06K,DS06V, DS06N
DS07B, DS07G,DS07R,DS07O,DS07K,DS07V, DS07N
Dyed Carboxyl polystyrene
Biomolecules may be covalently immobilized to carboxyl-functionalized microspheres. Our dyed carboxyl microspheres are impregnated with vibrant dyes for optimal visualization. See our color palette for a representation of our standard colors. Unless noted otherwise, they are supplied as a 5% solids suspension (w/v) and are available in four standard volumes: 0.5g, 1.0g, 1.5g, and 5.0g.
TechNote 205, Covalent Coupling
PDS 717, Visibly Dyed Microspheres
Catalog Number | Dye | Nominal Diameter | Specification Range |
---|---|---|---|
DCCB001 | Cabo Blue | 0.20µm | 0.190 - 0.210µm |
DCCR001 | Crimson Red | 0.20µm | 0.190 - 0.210µm |
DCSG001 | Shamrock Green | 0.20µm | 0.190 - 0.210µm |
DCBK001 | Basic Black | 0.20µm | 0.190 - 0.210µm |
DCCB002 | Cabo Blue | 0.50µm | 0.470 - 0.530µm |
DCCR002 | Crimson Red | 0.50µm | 0.470 - 0.530µm |
DCCB004 | Cabo Blue | 1.00µm | 0.95 - 1.05µm |
DCCR004 | Crimson Red | 1.00µm | 0.95 - 1.05µm |
DCTA004 | Tangerine Orange | 1.00µm | 0.95 - 1.05µm |
DCSG004 | Shamrock Green | 1.00µm | 0.95 - 1.05µm |
DCCB005 | Cabo Blue | 5.00µm | 4.80 - 5.20µm |
DCCR005 | Crimson Red | 5.00µm | 4.80 - 5.20µm |
DCBK005 | Basic Black | 5.00µm | 4.80 - 5.20µm |
References
Terao, Y, Takeshita, K, Nishiyama, Y, Morishita, N, Matsumoto, T, & Morimatsu, F. (2015) Promising Nucleic Acid Lateral Flow Assay Plus PCR for Shiga Toxin–Producing Escherichia coli. Journ of food protection, 78(8), 1560-1568.
Sheng, W, Li, S, Liu, Y, Wang, J, Zhang, Y, & Wang, S. (2017). Visual and rapid lateral flow immunochromatographic assay for enrofloxacin using dyed polymer microspheres and quantum dots. Microchimica Acta, 184(11), 4313-4321.(Dyed Black carboxyl microspheres)
DC03B, DC03G,DC03R,DC03O,DC03K,DC03V, DC03N
DC04B, DC04G,DC04R,DC04O,DC04K,DC04V, DC04N
DC05B, DC05G,DC05R,DC05O,DC05K,DC05V, DC05N
DC06B, DC06G,DC06R,DC06O,DC06K,DC06V, DC06N
DC07B, DC07G,DC07R,DC07O,DC07K,DC07V, DC07N
Dyed Protein-coated polystyrene
At times, our inventory includes streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.
PDS 721, Streptavidin Coated Microspheres
PDS 717, VIsibly Dyed Microspheres
Catalog Number | Coating / Dye | Nominal Diameter | Specification Range |
---|---|---|---|
CDCR001 | SA / Crimson Red | 0.20µm | 0.190 - 0.210µm |
CDCB001 | SA / Cabo Blue | 0.20µm | 0.190 - 0.210µm |
REFERENCES
Li S, Liu Y, Wang Y, Wang M, Liu C, & Wang Y. (2019) Rapid detection of Brucella spp. and elimination of carryover using multiple cross displacement amplification coupled with nanoparticles-based lateral flow biosensor. Frontiers in Cellular and Infection Microbiology, 9, 78. (Streptavidin Crimson Red nanoparticles [CP01R] for binding of biotinylated Brucella spp. amplicons in lateral flow biosensor.)