Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Estradiol [50-28-2]

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Cat# HY-B0141-1g

Size : 1g

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Telefono : +1 850 650 7790

Marca : MedChemExpress

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Description

Estradiol (β-Estradiol) is a steroid hormone and the major female sex hormone. Estradiol can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering^[1]^[2].

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	39.81 μM Compound: ES	Antiproliferative activity mouse B16F10 cells after 2 days by MTT assay Antiproliferative activity mouse B16F10 cells after 2 days by MTT assay	[PMID: 25222876]
CHO	IC₅₀	> 100 μM Compound: ES	Antiproliferative activity against CHO cells after 2 days by MTT assay Antiproliferative activity against CHO cells after 2 days by MTT assay	[PMID: 25222876]
CHO	EC₅₀	38.4 μM Compound: 55	Agonist activity at human TGR5 expressed in CHO cells by luciferase assay Agonist activity at human TGR5 expressed in CHO cells by luciferase assay	[PMID: 18307294]
CHO-K1	EC₅₀	0.03 μM Compound: E2	Agonist activity at ERbeta expressed in CHO-K1 cells by luciferase reporter gene transactivation assay Agonist activity at ERbeta expressed in CHO-K1 cells by luciferase reporter gene transactivation assay	[PMID: 20553023]
CHO-K1	EC₅₀	0.036 nM Compound: E2	Transcriptional activation of ERbeta receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay Transcriptional activation of ERbeta receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22647217]
CHO-K1	EC₅₀	0.05 nM Compound: E2	Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22647217]
CHO-K1	EC₅₀	0.05 μM Compound: E2	Agonist activity at ERalpha expressed in CHO-K1 cells by luciferase reporter gene transactivation assay Agonist activity at ERalpha expressed in CHO-K1 cells by luciferase reporter gene transactivation assay	[PMID: 20553023]
COS-7	EC₅₀	0.3 nM Compound: 17beta-estradiol	Agonist activity at GFP-tagged GPR30 expressed in COS7 cells assessed as half life for increase in intracellular calcium level by spectrofluorimetry Agonist activity at GFP-tagged GPR30 expressed in COS7 cells assessed as half life for increase in intracellular calcium level by spectrofluorimetry	[PMID: 16520733]
COS-7	EC₅₀	11 nM Compound: Estradiol	Agonist activity at estrogen receptor beta ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay Agonist activity at estrogen receptor beta ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay	[PMID: 19863083]
COS-7	EC₅₀	3.2 nM Compound: Estradiol	Agonist activity at estrogen receptor alpha ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay Agonist activity at estrogen receptor alpha ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay	[PMID: 19863083]
HEC-1	EC₅₀	0.09 nM Compound: Estradiol	Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 21481497]
HEC-1	EC₅₀	0.1 nM Compound: estradiol	Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay	[PMID: 19286283]
HEC-1	EC₅₀	0.123 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]
HEC-1	EC₅₀	0.13 nM Compound: estradiol	Agonist activity at full-length human estrogen receptor beta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at full-length human estrogen receptor beta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 19128016]
HEC-1	EC₅₀	0.16 nM Compound: estradiol	Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay	[PMID: 25559213]
HEC-1	EC₅₀	0.2 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]
HEC-1	EC₅₀	0.38 nM Compound: estradiol	Agonist activity at human ERbeta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay Agonist activity at human ERbeta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay	[PMID: 25559213]
HEC-1	EC₅₀	0.5 nM Compound: estradiol	Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay	[PMID: 19286283]
HEC-1	EC₅₀	0.7 nM Compound: estradiol	Agonist activity at full-length human estrogen receptor alpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at full-length human estrogen receptor alpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 19128016]
HEC-1	EC₅₀	0.72 nM Compound: Estradiol	Agonist activity at human full-length ERbeta receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at human full-length ERbeta receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 21481497]
HEK293	EC₅₀	0.024 nM Compound: beta-Est	Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HEK293	EC₅₀	0.046 nM Compound: 1	Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assay Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assay	[PMID: 21885279]
HEK293	EC₅₀	0.085 nM Compound: beta-Est	Agonist activity at human ERbeta expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human ERbeta expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HEK293	EC₅₀	0.09 nM Compound: E2	Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	0.13 nM Compound: E2	Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	0.75 nM Compound: estradiol	Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay	[PMID: 17890084]
HEK293	EC₅₀	0.75 nM Compound: estradiol	Agonist activity at human recombinant ERalpha expressed in HEK293 cells by transactivation assay Agonist activity at human recombinant ERalpha expressed in HEK293 cells by transactivation assay	[PMID: 17448656]
HEK293	EC₅₀	2.1 nM Compound: estradiol	Agonist activity at ERbeta expressed in HEK293 cells by transactivation assay Agonist activity at ERbeta expressed in HEK293 cells by transactivation assay	[PMID: 17890084]
HEK293	EC₅₀	2.1 nM Compound: estradiol	Agonist activity at human recombinant ERbeta expressed in HEK293 cells by transactivation assay Agonist activity at human recombinant ERbeta expressed in HEK293 cells by transactivation assay	[PMID: 17448656]
HEK293	EC₅₀	4.6 nM Compound: E2	Agonist activity at ZFP-fused ERRbeta LBD expressed in HEK293 cells by TR-FRET assay Agonist activity at ZFP-fused ERRbeta LBD expressed in HEK293 cells by TR-FRET assay	[PMID: 21218783]
HEK293	IC₅₀	50 μM Compound: Estradiol	Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit	[PMID: 22761000]
HEK-293T	EC₅₀	10.4 nM Compound: E2	Agonist activity at human ERbeta E305A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay Agonist activity at human ERbeta E305A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay	[PMID: 20659801]
HEK-293T	EC₅₀	2.5 nM Compound: E2	Agonist activity at human ERalpha E353A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay Agonist activity at human ERalpha E353A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay	[PMID: 20659801]
HeLa	EC₅₀	0.21 nM Compound: E2	Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HeLa	EC₅₀	0.37 nM Compound: E2	Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HeLa	EC₅₀	0.7 nM Compound: 38	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 37077378]
Hepatocyte	EC₅₀	1.9 μM Compound: Estradiol	Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method	[PMID: 22815312]
Hepatocyte	EC₅₀	4.1 μM Compound: Estradiol	Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method	[PMID: 22815312]
HepG2	EC₅₀	11 nM Compound: E2, estradiol	Agonist activity at human ERbeta expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay Agonist activity at human ERbeta expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22283328]
HepG2	EC₅₀	2.2 nM Compound: E2, estradiol	Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22283328]
Ishikawa	EC₅₀	0.047 nM Compound: 17-beta-estradiol	Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolism of p-nitrophenol phosphatase after 72 hrs Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolism of p-nitrophenol phosphatase after 72 hrs	[PMID: 15568770]
Ishikawa	EC₅₀	0.9 nM Compound: E2	Agonist activity at estrogen receptor in human Ishikawa cells assessed as increase of alkaline phosphatase level after 3 days using P-nitrophenyl phosphate as substrate Agonist activity at estrogen receptor in human Ishikawa cells assessed as increase of alkaline phosphatase level after 3 days using P-nitrophenyl phosphate as substrate	[PMID: 22178552]
Ishikawa	EC₅₀	24.6 pM Compound: E2	Estrogenic activity at estrogen receptor in human Ishikawa cells assessed as induction of alkaline phosphatase expression after 72 hrs by microplate reader Estrogenic activity at estrogen receptor in human Ishikawa cells assessed as induction of alkaline phosphatase expression after 72 hrs by microplate reader	[PMID: 22464681]
JEG-3	IC₅₀	84000 nM Compound: 3	Estrone sulfatase activity against homogenized human JEG-3 cells was determined by measuring the [3H]E1 obtained from [3H]E1S Estrone sulfatase activity against homogenized human JEG-3 cells was determined by measuring the [3H]E1 obtained from [3H]E1S	[PMID: 9873454]
MCF7	EC₅₀	0.0084 nM Compound: estradiol	Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCR Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCR	[PMID: 25559213]
MCF7	EC₅₀	0.01 nM Compound: 1 (17-beta-estradiol)	Effective concentration against MCF-7 breast tumor cells using MCF-7 assay. Effective concentration against MCF-7 breast tumor cells using MCF-7 assay.	[PMID: 12825935]
MCF7	EC₅₀	0.3 nM Compound: estradiol	Agonist activity at human ERbeta in human MCF7 cells assessed as otubain 2 endogenous genes activation after 24 hrs by qPCR Agonist activity at human ERbeta in human MCF7 cells assessed as otubain 2 endogenous genes activation after 24 hrs by qPCR	[PMID: 25559213]
MCF7	IC₅₀	0.4 nM Compound: estrogen	Inhibition of chemoattractant-induced cell migration of human MCF7 cells after 48 hrs by transwell migration assay Inhibition of chemoattractant-induced cell migration of human MCF7 cells after 48 hrs by transwell migration assay	[PMID: 16520733]
MCF7	EC₅₀	10 nM Compound: E2	Agonist activity at human ERalpha LBD in human MCF7 cells assessed as induction of cell proliferation after up to 6 days by celltiter-glo assay Agonist activity at human ERalpha LBD in human MCF7 cells assessed as induction of cell proliferation after up to 6 days by celltiter-glo assay	[PMID: 21218783]
MCF7	IC₅₀	27.35 μM Compound: ES	Antiproliferative activity human ER-positive MCF7 cells after 2 days by MTT assay Antiproliferative activity human ER-positive MCF7 cells after 2 days by MTT assay	[PMID: 25222876]
MCF7	EC₅₀	3.7 x 10^-12 M Compound: estradiol	Estrogenic activity in human MCF7 cells assessed as cell proliferation after 6 days by WST8 assay Estrogenic activity in human MCF7 cells assessed as cell proliferation after 6 days by WST8 assay	[PMID: 18760603]
MCF7	IC₅₀	32 μM Compound: estradiol	Inhibitory concentration against MCF-7 (ER+) cell line using SRB assay Inhibitory concentration against MCF-7 (ER+) cell line using SRB assay	[PMID: 12565971]
MCF7	EC₅₀	6.4 pM Compound: E2	Antiproliferative activity against estrogen-deficient human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against estrogen-deficient human MCF7 cells after 72 hrs by MTT assay	[PMID: 22464681]
MCF7	EC₅₀	8.0 x 10^-11 M Compound: estradiol	Effective concentration against human ER positive MCF-7 breast cancer cell line evaluated by luciferase assay Effective concentration against human ER positive MCF-7 breast cancer cell line evaluated by luciferase assay	[PMID: 16190777]
MDA-MB-231	IC₅₀	> 100 μM Compound: estradiol	Inhibitory concentration against MDA-MB-231 (ER-) cell line using SRB assay Inhibitory concentration against MDA-MB-231 (ER-) cell line using SRB assay	[PMID: 12565971]
MDA-MB-231	IC₅₀	33.46 μM Compound: ES	Antiproliferative activity human ER-negative MDA-MB-231 cells after 2 days by MTT assay Antiproliferative activity human ER-negative MDA-MB-231 cells after 2 days by MTT assay	[PMID: 25222876]
MIA PaCa-2	IC₅₀	42.93 μM Compound: ES	Antiproliferative activity human MIAPaCa2 cells after 2 days by MTT assay Antiproliferative activity human MIAPaCa2 cells after 2 days by MTT assay	[PMID: 25222876]
NIH3T3	IC₅₀	> 100 μM Compound: ES	Antiproliferative activity mouse NIH/3T3 cells after 2 days by MTT assay Antiproliferative activity mouse NIH/3T3 cells after 2 days by MTT assay	[PMID: 25222876]
SAOS-2	ED₅₀	4.7 nM Compound: 2	Functional Estrogen receptor beta activity by metallothionein induction in SAOS-2 cells Functional Estrogen receptor beta activity by metallothionein induction in SAOS-2 cells	[PMID: 12824043]
SK-BR-3	IC₅₀	0.1 nM Compound: estrogen	Inhibition of chemoattractant-induced cell migration of human SK-BR-3 cells after 48 hrs by transwell migration assay Inhibition of chemoattractant-induced cell migration of human SK-BR-3 cells after 48 hrs by transwell migration assay	[PMID: 16520733]
U2OS	EC₅₀	0.004 nM Compound: E2	Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control	[PMID: 23043242]
U2OS	EC₅₀	0.01 nM Compound: E2	Activation of human ERbeta expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control Activation of human ERbeta expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control	[PMID: 23043242]
U2OS	EC₅₀	0.019 nM Compound: estradiol	Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT	[PMID: 16162002]
U2OS	EC₅₀	0.098 nM Compound: estradiol	Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT	[PMID: 16162002]
U2OS	EC₅₀	0.15 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]
U2OS	EC₅₀	0.4 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]

In Vitro

Estradiol (10 nM, 7 days) induces neural differentiation and increased neurite branching of human endometrial stem cells (hEnSCs)^[1].
Estradiol (17β-estradiol, 10 nM, 7 days) increases the expression of neuron-like cell markers (Tuj-1, nestin and NF-H) in neural-like cells differentiated from hEnSCs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[1]

Cell Line:	Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration:	10 nM
Incubation Time:	7 days
Result:	Increased the number of neurite processes including neural differentiation and neurite branching.

Immunofluorescence^[1]

Cell Line:	Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration:	10 nM
Incubation Time:	7 days
Result:	Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.

In Vivo

Estradiol (1 nM, the hippocampal slices from FBN-ARO-KO mice) rescues long-term potentiation (LTP) amplitude^[1].
Estradiol (0.0167 mg, implanted s.c., FBN-ARO-KO mice) rescues the molecular and functional deficits in FBN-ARO-KO mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	FBN-ARO-KO Mice^[2]
Dosage:	1 nM
Administration:	Treated for the hippocampal slices
Result:	Rescued long-term potentiation (LTP) amplitude of both male and female mice.

Animal Model:	FBN-ARO-KO Mice^[2]
Dosage:	0.0167 mg
Administration:	Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation.
Result:	Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain. Rescued the spatial learning and memory defects.

Essai clinique

Masse moléculaire

272.38

Formule

C₁₈H₂₄O₂

CAS No.

50-28-2

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1([C@H]2O)[C@](CC2)(07)[C@@](CCC3=C4C=CC(O)=C3)(07)[C@]4(07)CC1

Structure Classification

Steroids

Initial Source

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (367.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 20 mg/mL (73.43 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6713 mL	18.3567 mL	36.7134 mL
5 mM	0.7343 mL	3.6713 mL	7.3427 mL
10 mM	0.3671 mL	1.8357 mL	3.6713 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 2.5 mg/mL (9.18 mM); Suspended solution; Need ultrasonic
Protocol 3

Add each solvent one by one: 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
Protocol 4

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Corn Oil
Solubility: 12.5 mg/mL (45.89 mM); Suspended solution; Need ultrasonic

Pureté et documentation

Purity: 99.93%

Fiche technique (279 KB) SDS (563 KB)

COA (249 KB) HNMR (280 KB) LCMS (87 KB) Elemental Analysis Report (107 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Elham Hasanzadeh. Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells. Cell Biol Int. 2021 Jan;45(1):140-153. [Content Brief]

[2]. Yujiao Lu, et al. Neuron-Derived Estrogen Regulates Synaptic Plasticity and Memory. J Neurosci. 2019 Apr 10;39(15):2792-2809. [Content Brief]

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