ExoBrite™ CD9 Flow Antibody is validated by Biotium for optimal detection of EV marker CD9 in purified or bead-bound EVs by flow cytometry. The antibody conjugates offer exceptional signal-to-noise and include color options for the Pacific Blue™, FITC, PE, and APC channels.

• NEW: Now with updated buffer formulation for improved signal-to-noise
• Optimal detection of EV marker CD9 by flow cytometry
• Validated for purified or bead-bound EVs
• Bright signal and low background
• Available in 4 colors

The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.

EV antibodies you can trust

Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise. ExoBrite™ Isotype Control Flow Antibodies, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.

For general EV staining, Biotium’s ExoBrite™ True EV Membrane Stains offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.

Biotium also offers conjugated ExoBrite™ Western Antibodies against CD9, CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.

Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.

ExoBrite™ Flow Antibody Conjugates