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> Fab Mouse IgG (H&L) antibody (TRITC)

Fab Mouse IgG (H&L) antibody (TRITC)

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Cat# orb348470-1mg

Size : 1mg

Marca : Biorbyt

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    Fab Mouse IgG (H&L) antibody (TRITC)

    Catalog Number: orb348470

    Catalog Numberorb348470
    CategoryAntibodies
    DescriptionFab Mouse IgG (H&L) antibody (TRITC)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsDOT, FC, FLISA, IF
    ReactivityMouse
    IsotypeIgG Fab
    ImmunogenMouse IgG whole molecule
    Concentration1.0 mg/mL
    Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
    ConjugationTRITC
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesGoat Fab Anti-Mouse IgG Antibody Rhodamine Conjuga
    NoteFor research use only
    Application notesFab Anti-Mouse IgG (H&L) Rhodamine Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Expiration Date12 months from date of receipt.
    Fab Mouse IgG (H&L) antibody (TRITC)

    Dot Blot results of Goat Fab Anti-Mouse IgG Antibody Rhodamine Conjugated. Dots are Mouse IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Mouse IgG TRITC at 1 µg/mL for 1 hr at RT.