Fast Mutagenesis System
Cat# FM111-01
Size : 10rxns
Marca : TransGen Biotech
Contatta il distributore locale :
Telefono : +1 850 650 7790
Manual• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.
• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.
• Mutation sites on both primers to improve mutation efficiency.
• Partially overlapping primers for exponential DNA amplification.
• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.
• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.
Storage
Competent Cell at -70°C for six months; others at -20°C for two years
Shipping
Dry ice (-70℃)
| Component | FM111-01 (10 rxns) | FM111-02 (20 rxns) |
| 2×TransStart® FastPfu Fly PCR SuperMix | 250 μl | 500 μl |
| DMT Enzyme (10 units/μl) | 10 μl | 20 μl |
| DMT Chemically Competent Cell | 10×50 μl | 20×50 μl |
| Nuclease-free Water | 1 ml | 1 ml |
| SControl Plasmid (5 ng/μl) | 10 μl | 20 μl |
| SControl Primers (10 μM ) | 10 μl | 20 μl |
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