FBL ELISA Kit (Human)
Cat# OKWB00172
Size : 96Wells
Marca : Aviva Systems Biology
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Telefono : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for FBL ELISA Kit (Human) (OKWB00172) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology FBL ELISA Kit (Human) (OKWB00172) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FBL has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FBL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FBL captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 1.563-100ng/ml | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.938 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Type: Quantitative Sandwich ELISA |
| Gene Symbol | FBL |
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| Gene Full Name | fibrillarin |
| Alias Symbols | 34 kDa nucleolar scleroderma antigen;34-kD nucleolar scleroderma antigen;FIB;FLRN;histone-glutamine methyltransferase;Nop1;RNA, U3 small nucleolar interacting protein 1;RNU3IP1;rRNA 2'-O-methyltransferase fibrillarin;U6 snRNA 2'-O-methyltransferase fibrillarin. |
| NCBI Gene Id | 2091 |
| Protein Name | rRNA 2'-O-methyltransferase fibrillarin |
| Description of Target | S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). |
| Uniprot ID | P22087 |
| Protein Accession # | NP_001427 |
| Nucleotide Accession # | NM_001436 |