GAPDH

Cat# NB-26-01998

Size : 100ul

Marca : Neo Biotech

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Product Details
Applications: ICC, WB, IHC, IF
Type: Mouse IgM
Dilutions: Immunofluorescence: 1:100; Immunohistochemistry: 1:100; Western Blot: 1:1,000
Immunogen: Full length protein purified from pig blood cells
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Chicken, Bovine, Dog, Porcine, Equine
Entrez:
UniProt:
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to GAPDH, MO22204, dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. MO22204 produces strong cytoplasmic staining of healthy cells.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to GAPDH, MO22204, dilution 1:100 in green. Blue is Hoechst staining of nuclear DNA. MO22204 produces strong cytoplasmic staining of healthy cells.

Western Blot

Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, MO22204, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.

Western Blot

Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, MO22204, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.