AP1489a-ev | GLUT2 (SLC2A2) Antibody (N-term) Clinisciences

Western blot analysis of SLC2A2 Antibody (N-term) (Cat.#AP1489a) in HepG2 cell line lysates (35ug/lane). SLC2A2 (arrow) was detected using the purified Pab.
Anti-SLC2A2 Antibody (N-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Flow cytometric analysis of HL-60 cells using GLUT2 (SLC2A2) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	FC, WB, E
Primary Accession	P11168
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	57490 Da
Antigen Region	31-60 aa

Additional Information
Gene ID	6514
Other Names	Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver, GLUT-2, SLC2A2, GLUT2
Target/Specificity	This GLUT2 (SLC2A2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-60 amino acids from the N-terminal region of human GLUT2 (SLC2A2).
Dilution	WB~~1:1000 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GLUT2 (SLC2A2) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SLC2A2 (HGNC:11006)
Function	Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose (PubMed:16186102, PubMed:23396969, PubMed:28083649, PubMed:8027028, PubMed:8457197). Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell (PubMed:8027028). May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney (PubMed:3399500). Also able to mediate the transport of dehydroascorbate (PubMed:23396969).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Liver, insulin-producing beta cell, small intestine and kidney.

GLUT2 (SLC2A2) Antibody (N-term)