Glyceraldehyde 3-Phosphate Dehydrogenase (GAPD, GAPDH) (Glycerol Free) (HRP)

Cat# G8140-01-HRP-100ul

Size : 100ul

Marca : US Biological

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G8140-01-HRP Glyceraldehyde 3-Phosphate Dehydrogenase (GAPD, GAPDH) (HRP)

Clone Type
Polyclonal
Host
mouse
Source
rabbit
Swiss Prot
P04406
Isotype
IgG1
Grade
Affinity Purified
Applications
E IC IF WB
Crossreactivity
Ca Fe Hu Mo Po Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the|beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).||Applications:|Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.||Recommended Dilutions:|Western Blot: Detects on 10ug of A431 lysates.|Immunocytochemistry: Dilute in PBS, 10mg/ml BSA. Fixative: 4% PFA, 0.02% PBS-Triton X-100. Incubation time: 3 minutes at RT.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.     ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q70 (6C5)|Host: mouse|Source: rabbit|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography|Immunogen: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle. |Specificity: Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle at ~38kD. Also recognizes cardiac GAPDH. Species Crossreactivity: human, porcine, canine, feline, rat, mouse and fish. Does not react with GAPDH from E. coli. Also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle at ~38kD. Also recognizes cardiac GAPDH. Species Crossreactivity: human, porcine, canine, feline, rat, mouse and fish. Does not react with GAPDH from E. coli. Also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.
References
1. Liu, S. et al., (2010) Acta Physiol (Oxf) 199; 339–346. 2. Fatima, J. et al., (2008) J. Gastrointestinal Surgery 13:634-641. 3. Iqbal, C.W. et al., (2008) J. Gastrointestinal Surgery 12: 1854-1865. 4. Qandeel, H.G. et al., (2009) J. Surgical Research 156:123-128. 5. Iqbal, C. W. et al. (2009) Surgery 146:100-112. 6. Houghton, S.G. et al., (2008) Surgery 143:79-93. 7. Houghton, S.G. et al., (2006) Surgery 139:542-549. 8. Hwang, Y.S. et al., (2006) Mol Carcinog. 45:518-5129. 9. Chui, Y.-L. et al., (2010) Biochem. Biophys. Res. Comm. 391; 1522-1525. 10. Saha, A. et al., (2012) PLoS Pathog 8(3): e1002573. doi:10.1371/journal.ppat.1002573. 11. Banerjee, S. et al., (2013) PLoS Pathogens 9(5): e1003314. doi:10.1371/journal.ppat.1003314. 1. Almeras, Linoel, et al. Proteomics. 7:769-80 (2007). 2. Kaltenbach, Linda S., et al. PLoS Genet. 3:e82 (2007). 3. Donofrio, Gaetano, et al. Microbes Infect. 8:898-904 (2006). 4. Chen, L., et al. Mole. Vision. 9:151-158 (2003). 5. Law J.W.S., Lee A.Y.W., Sun M., Nikoenko & Morellini F. J Neurosci. 23(32): 10419-10432 (2003).