Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Anti-PSD-95 Antibody FL490 Conjugate

Stampa

Cat# 75-028-FL490

Size : 200uL

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : Antibodies Incorporated

Contatta il distributore locale :

Telefono : +1 850 650 7790

Our Anti-PSD 95 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Species Reactivity

Chicken, Human, Mouse, Non-Human Primate, Rat

Applications

ICC, IHC

Host Species

Mouse

Available Conjugates

Unconjugated

550

594

650

Data Sheet

KO Validated

SKU: 75-028-FL490

Product Details

Target

PSD 95

Target Description

PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Format

Purified by Protein A chromatography

Concentration

0.5 mg/mL

Clonality

Monoclonal

Clone

K28/43

Isotype

IgG2a

Applications

ICC, IHC

Host Species

Mouse

Gene Name

DLG4 PSD95

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Antigen

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

Species Reactivity

Chicken, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2939168

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

Specificity

No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

UniProt Details

UniProt (Human): P78352
UniProt (Immunogen Species): P78352

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