H2DCFDA [4091-99-0]
Cat# HY-D0940-50mg
Size : 50mg
Marca : MedChemExpress
H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm).
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H2DCFDA Chemical Structure
CAS No. : 4091-99-0
This product is a controlled substance and not for sale in your territory.
Based on 323 publication(s) in Google Scholar
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Mar 10;e2103317. [Abstract]
- CLSM images of HUVEC cells using DCFH‐DA (0.5 hours) as the fluorescent probe: Control, H2O2, H2O2+10 µg/mL1 PDA NPs, H2O2+40 µg/mL PDA NPs, H2O2+80 µg/mL PDA NPs.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 16 July 2022.
- Treated HL-1 cells are washed three times and incubated with DMEM medium containing H2DCFDA (10 μM; 30 min) at 37 °C. The cells then are washed three times with warm DMEM medium and intracellular ROS production is detected by flow cytometry.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Jul 21;287:121688. [Abstract]
- CLSM images of 4T1 tumor cells stained with DCFH-DA (15 min) after different treatments.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 May;284:121513. [Abstract]
- DCFH-DA (10 μM; 30 min). The ROS generation in 4T1 cells and NIH-3T3 cells incubated with various formulations for 4 h observed by CLSM.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Sep 30;290:121842. [Abstract]
- DCFH-DA (10 μM; 30 min) or C11-BODIPY-labeled cells are substantially increased after treatment with 1 μM FeSO4.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2022 Oct 5;439:129502. [Abstract]
- Representative DCFH-DA (10 μM; 30 min) staining images (Green) in PBNs exposed PSNPs and/or LPS in vivo and in vitro.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Cell. 2021 Jun 24;184(13):3528-3541.e12. [Abstract]
- Stained protoplasts with H2DCFDA for the detection of ROS production and PI for PM integrity and tracked the process by live-cell imaging.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: ACS Nano. 2021 Oct 26;15(10):16286-16297. [Abstract]
- CLSM image of 4T1 cells after being treated with MM, MMP, and MMTP; cells are stained with DCFH-DA (20 μM; for 1 h).
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Biomaterials. 2021 Oct;277:121103. [Abstract]
- In vitro experiments are conducted to measure the cellular ROS levels in A549 cells from different treatments via flow cytometry using fluorescent probe H2DCFDA (5 μM; 30 min) to better understand the potential molecular mechanism of SOD@ARA290-HBc on radiation-induced lung injury. H2DCFDA fluorescence intensity in A549 cells significantly increased after irradiation.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Small. 2021 Aug;17(32):e2101368. [Abstract]
- Laser scanning confocal fluorescence microscopy images of HT-1080 cells treated with different nanodrugs for the detection of intracellular lipid peroxidation and total ROS using C11-BODIPY and DCFH-DA (100 nM; 10 min) as the corresponding fluorescence probes.
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H2DCFDA purchased from MedChemExpress. Usage Cited in: Curr Biol. 2021 May 18;S0960-9822(21)00607-2. [Abstract]
- Co-staining stigmas with H2DCFDA (50 μM; for 1-2 hours) and the cell wall indicator propidium iodide (PI) shows that ROS is located in the cytoplasm of papilla cells, near the periphery of the plasma membrane.
Description |
H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)[1]. |
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In Vitro |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
487.29 |
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Formule |
C24H16Cl2O7 |
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CAS No. |
4091-99-0 |
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Appearance |
Solid |
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Color |
White to off-white |
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Emission (Em) |
517 |
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Excitation (Ex) |
492 |
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SMILES |
O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=C(Cl)C(OC(C)=O)=C4)C=C(OC(C)=O)C(Cl)=C3 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
-20°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvant et solubilité |
In Vitro:
DMSO : 125 mg/mL (256.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Ethanol : 20 mg/mL (41.04 mM; Need ultrasonic) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation | |||||||||||||
Dyeing Example |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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Ethanol / DMSO | 1 mM | 2.0522 mL | 10.2608 mL | 20.5217 mL | 51.3042 mL |
5 mM | 0.4104 mL | 2.0522 mL | 4.1043 mL | 10.2608 mL | |
10 mM | 0.2052 mL | 1.0261 mL | 2.0522 mL | 5.1304 mL | |
15 mM | 0.1368 mL | 0.6841 mL | 1.3681 mL | 3.4203 mL | |
20 mM | 0.1026 mL | 0.5130 mL | 1.0261 mL | 2.5652 mL | |
25 mM | 0.0821 mL | 0.4104 mL | 0.8209 mL | 2.0522 mL | |
30 mM | 0.0684 mL | 0.3420 mL | 0.6841 mL | 1.7101 mL | |
40 mM | 0.0513 mL | 0.2565 mL | 0.5130 mL | 1.2826 mL | |
DMSO | 50 mM | 0.0410 mL | 0.2052 mL | 0.4104 mL | 1.0261 mL |
60 mM | 0.0342 mL | 0.1710 mL | 0.3420 mL | 0.8551 mL | |
80 mM | 0.0257 mL | 0.1283 mL | 0.2565 mL | 0.6413 mL | |
100 mM | 0.0205 mL | 0.1026 mL | 0.2052 mL | 0.5130 mL |
H2DCFDA Related Classifications
- Others
- Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
- Reactive Oxygen Species