HRP Dilution Buffer for Antibody Conjugates
Catalog Number: B2010080 (50 mL)
HRP-conjugated Antibody Dilution Buffer is a very stable and reproducible diluent for antibodies conjugated to HRP and used in ELISA and similar immuno applications. Buffer is specially formulated to optimize the activity, the stability and the reproducibility of the HRP-generated signal. Buffer contains Thimerosal as a preservative as all as proprietary proteins that minimize non-specific binding. Custom bulk orders are available upon request.
Product Description
HRP-conjugated Antibody Dilution Buffer
| Catalog number: | B2010080 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume/Weight | 50 mL |
| pH | 7.4 |
| Supplied as | Ready-to-use solution |
| Appearance | Clear solution |
| Applications | Dilution of HRP-conjugated antibodies used in ELISA and similar immunoapplications. Buffer does NOT contain sodium azide (inhibitor of HRP). |
| Shelf-Life | 1 year from date of manufacture |
| Storage: | Keep at 2-8°C |
| Keywords: | HRP diluent, HRP conjugate diluent, HRP-antibody conjugate diluent, diluent for antibody conjugated to HRP |
| Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. |
| References | 1: Ge M, Li RC, Qu T, Gong W, Yu XL, Tu C. Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies. AMB Express. 2017 Sep 18;7(1):177. doi: 10.1186/s13568-017-0473-3. PMID: 28921455; PMCID: PMC5603472.2: Poolperm P, Varinrak T, Kataoka Y, Tragoolpua K, Sawada T, Sthitmatee N. Development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera. J Microbiol Methods. 2017 Nov;142:10-14.3: Panda R, Boyer M, Garber EAE. A multiplex competitive ELISA for the detection and characterization of gluten in fermented-hydrolyzed foods. Anal Bioanal Chem. 2017 Dec;409(30):6959-6973. 4: Jadhav PD, Shim YY, Reaney MJ. Synthesis and Characterization of Site- Selective Orbitide-BSA Conjugate to Produce Antibodies. Bioconjug Chem. 2016 Oct 19;27(10):2346-2358. 5: Kim WJ, Cho HY, Jeong B, Byun S, Huh J, Kim YJ. Synergistic Use of Gold 6: Mitsui Y. Development of a simple and specific direct competitive ELISA for 7: Al-Dujaili EA, Baghdadi HH, Howie F, Mason JI. Validation and application of 8: Al-Dujaili EA. Development and validation of a simple and direct ELISA method |
| Products Realted to HRP Dilution Buffer | Biochemical Buffers and Solutions |
Additional Information
| Weight | 2.6 oz |
|---|---|
| Dimensions | 3.3 × 1.2 × 1.2 in |