Application
| WB, ICC |
|---|---|
| Primary Accession | P50454.2 |
| Other Accession | NP_001193943 |
| Host | Mouse |
| Isotype | IgG1 Kappa |
| Reactivity | Human |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human HSP47 Monoclonal IgG1 Kappa |
| Target/Specificity | Detects 47kDa. |
| Other Names | SerpinH1 Antibody, Colligin Antibody, Gp46 Antibody, serine proteinase inhibitor Antibody, cysteine proteinase inhibitor Antibody, collagen binding protein Antibody |
| Clone Names | 1C4-1A6 |
| Immunogen | Human HSP47, full length |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-203 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Endoplasmic Reticulum | Endoplasmic Reticulum Lumen |
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Provided below are standard protocols that you may find useful for product applications.
Background
HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions (1). The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).
References
1. Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212.
2. Taguchi T., et al. (2011) Acta Histochem Cytochem. 44(2):35-41.
3. Nong Z., et al. (2011) Am J Pathol. 197(5): 2189-96.