Human GM-CSF protein (active)

Cat# GTX137974-pro

Size : 100ug

Marca : GeneTex

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Human GM-CSF protein (active). GTX137974-pro
Human GM-CSF protein (active). GTX137974-pro

Application

Functional Assay

Species

Human
Package
100 μg
View product citations for antibody GTX137974-pro on CiteAb
PRODUCT

Summary

GM-CSF, a colony stimulating factor also known as CSF2, mediates the proliferation, differentiation, and functions of hematopoietic precursor cells. This bioactive recombinant human GM-CSF is a 14.6 kDa globular protein formed by a 128 a.a. polypeptide with two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

APPLICATION

Application Note

The ED₅₀ was determined by a cell proliferation assay using TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Observed MW

14 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1 mg/ml. Lyophilized from 50mM PBS, 100mM NaCl, 5% Trehalose.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

Human full-length GM-CSF protein (18-144 a.a. of NP_000749.2, signal peptide removed)

Expression System

E. Coli

Purity

>98%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

colony stimulating factor 2 , CSF , GMCSF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Human GM-CSF protein (active). GTX137974-pro

GTX137974-pro Functional Assay Image

Serial dilutions of Human GM-CSF protein (active) were added to TF-1 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

*This product has been tested against the competitor and the biological activity is better than competitor.

Human GM-CSF protein (active). GTX137974-pro

GTX137974-pro Image

1μg of GTX137974-pro Human GM-CSF protein (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

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REVIEW

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