EA100949 | Human Herpes Simplex Virus type 2 (HSV-2) IgG ELISA kit

Human Herpes Simplex Virus type 2 (HSV-2) IgG ELISA kit

SKU

EA100949

Human Herpes Simplex Virus type 2 (HSV-2) IgG ELISA kit

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Datasheet SDS

3 Weeks*

Specifications
Product Data
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human Herpes Simplex Virus type 2 (HSV-2) IgG
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with HSV-2 antigen: 12x8x1 2. Sample Diluent: 1 bottle (ready to use): 22 ml 3. Calibrator: yellow Cap. 1 Vial (ready to use): 1ml 4. Positive Control: Red Cap. 1 vial (ready to use): 1ml 5. Negative Control: Blue Cap. 1 vial (ready to use): 1ml 6. Enzyme conjugate: 1 bottle (ready to use): 12ml 7. TMB Substrate: 1 bottle (ready to use): 12ml 8. Stop Solution: 1 bottle (ready to use): 12ml 9. Wash concentrate 20X: 1 bottle: 25ml
Background	HSV-1 and 2 are virtually identical, sharing approximately 50% of their DNA and have over 80% of common antigens. Both types infect the body's mucosal surfaces, usually the mouth or genitals, and then establish latency in the nervous system. For both types, at least two-thirds of infected people have no symptoms, or symptoms too mild to notice. However, both types can recur and spread even when no symptoms are present. By the time they're teenagers or young adults, about 50% of Americans have HSV-1 antibodies in their blood. By the time they are over age 50, some 80-90% of Americans has HSV-1 antibodies. By comparison, almost all HSV-2 is encountered after childhood, when people become sexually active. HSV type 1 is the cause of most orofacial herpes and HSV encephalitis; type 2 is the primary cause of initial and recurrent genital herpes and neonatal HSV. Reactivation of latent HSV infection is a frequent complication of immunosuppression due to cancer, transplantation and AIDS. Asymptomatic genital shedding of HSV-2 is more common than HSV-1 and occurs more frequently during the first 3 months after acquisition of primary type 2 disease than during later periods. The presence of HSV IgG antibody is indicative of previous exposure A significant increases in HSV IgG is an indicative of reactivation, current or recent infection. IgM antibody is present after primary HSV infection.

Specifications

Product Data

Format

8x12 divisible strips

Assay Type

Solid Phase Sandwich ELISA

Assay Length

3 hours

Signal

Colorimetric

Sample Type

Serum

Sample Volume

10 µl/well

Specificity

This kit is used for quantitative detection human Herpes Simplex Virus type 2 (HSV-2) IgG

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Interference

No significant interference observed with available related molecules.

Components

1. Microwells coated with HSV-2 antigen: 12x8x1
2. Sample Diluent: 1 bottle (ready to use): 22 ml
3. Calibrator: yellow Cap. 1 Vial (ready to use): 1ml
4. Positive Control: Red Cap. 1 vial (ready to use): 1ml
5. Negative Control: Blue Cap. 1 vial (ready to use): 1ml
6. Enzyme conjugate: 1 bottle (ready to use): 12ml
7. TMB Substrate: 1 bottle (ready to use): 12ml
8. Stop Solution: 1 bottle (ready to use): 12ml
9. Wash concentrate 20X: 1 bottle: 25ml

Background

HSV-1 and 2 are virtually identical, sharing approximately 50% of their DNA and have over 80% of common antigens. Both types infect the body's mucosal surfaces, usually the mouth or genitals, and then establish latency in the nervous system. For both types, at least two-thirds of infected people have no symptoms, or symptoms too mild to notice. However, both types can recur and spread even when no symptoms are present. By the time they're teenagers or young adults, about 50% of Americans have HSV-1 antibodies in their blood. By the time they are over age 50, some 80-90% of Americans has HSV-1 antibodies. By comparison, almost all HSV-2 is encountered after childhood, when people become sexually active. HSV type 1 is the cause of most orofacial herpes and HSV encephalitis; type 2 is the primary cause of initial and recurrent genital herpes and neonatal HSV. Reactivation of latent HSV infection is a frequent complication of immunosuppression due to cancer, transplantation and AIDS. Asymptomatic genital shedding of HSV-2 is more common than HSV-1 and occurs more frequently during the first 3 months after acquisition of primary type 2 disease than during later periods. The presence of HSV IgG antibody is indicative of previous exposure A significant increases in HSV IgG is an indicative of reactivation, current or recent infection. IgM antibody is present after primary HSV infection.

Human Herpes Simplex Virus type 2 (HSV-2) IgG ELISA kit

Cat# EA100949

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Human Herpes Simplex Virus type 2 (HSV-2) IgG ELISA kit

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