Human IL-4 ELISA Kit (96-well)
Cat# EA100022
Size : 1x96wells
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| Product Data | |
| ACCN | NM_000589 |
|---|---|
| Assay Length | 3 hours |
| Assay Platform | 96-well plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Type | Sandwich |
| Background | Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils . The sequence of human IL-4 cDNA predicts a 153 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to form the mature protein . At the amino acid sequence level, mature human IL-4 is approximately 50% identical to mouse IL-4 and there is no species cross-reactivity between the two proteins . Human IL-4 also shares approximately 30% amino acid sequence identity to human IL-13 and the two cytokines exhibit overlapping biological activities . The gene for IL-4 has been mapped to human chromosome 5q, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF . IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the TH2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro . The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15 . A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the more recently cloned IL-13 R , has also been proposed . Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 . In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates . Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution. |
| Components |
|
| Curve Range | 7.8-500pg/ml |
| figure_legend | Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent. |
| Format | 96-well strip plate |
| Gene Symbol | IL4 |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 3565 |
| Reactivity | Human |
| Reactivity | Human |
| Reactivity | Human |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Sensitivity | 2pg/mL |
| Signal | Colorimetric |
| Species | Human |
| Specificity | Natural and recombinant Human IL-4 Ligand |
| Standard Curve | /assets/images/elisa/kits/128/che0005-096-1-e.jpg |
| Target Symbol | IL4 |
| Description | Human IL-4 ELISA Kit (96-well) |