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> IgA, alpha (FITC)

IgA, alpha (FITC)

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Cat# I1890-05K-1mg

Size : 1mg

Marca : US Biological

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I1890-05K IgA, alpha (FITC)

Clone Type
Polyclonal
Host
goat
Source
rat
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IF WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications: |Suitable for use in Immunfluorescence and FLISA. Other applications have not been tested.||Recommended Dilutions: |Immunofluorescence Microscopy: 1:1000-1:5000|FLISA: 1:10,000-1:50,000|Optimal dilutions to be determined by the researcher.||Absorption Wavelength: 495nm||Emission Wavelength: 528nm||Fluorochrome/Protein Ratio: |9.4 moles FITC per mole of Goat IgG||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC) (MW: 390 daltons). Reconstitute in 1ml sterile ddH2O. |Purity: Purified by immunoaffinity chromatography .|Immunogen: Rat IgA alpha heavy chain|Specificity: Recognizes rat IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgA and rat serum. Shows <1% crossreactivity in ELISA against other rat heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgA alpha heavy chain
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC) (MW: 390 daltons). Reconstitute in 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography .
Specificity
Recognizes rat IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgA and rat serum. Shows <1% crossreactivity in ELISA against other rat heavy or light chain isotypes.