IL23A ELISA Kit (Sheep)

Cat# OKWB00432

Size : 96Wells

Marca : Aviva Systems Biology

Richiedere ulteriori informazioni

Contatta il distributore locale :


Telefono : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for IL23A ELISA Kit (Sheep) (OKWB00432)
Product Info
Predicted Species ReactivityOvis aries|Sheep
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-104%86-104%87-102%89-102%
EDTA Plasma (n=5)89-100%82-101%83-97%88-97%
Heparin Plasma (n=5)89-97%81-97%80-99%82-97%
ELISA Kit PrincipleAviva Systems Biology IL23a ELISA Kit (Sheep) (OKWB00432) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL23a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL23a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL23a captured in the well.
ELISA Kit Range15.625-1000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)91-10499
EDTA Plasma (n=5)86-9890
Heparin Plasma (n=5)86-10195
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
IL23a Microplate96 Wells (12 x 8 Well strips)
IL23a Lyophilized Standard2 x 1 ng
100X IL23a Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity9.4 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolIL23A
Gene Full Nameinterleukin 23 subunit alpha
Alias Symbolsinterleukin 23 subunit p19;interleukin 23, alpha subunit p19;interleukin-23 subunit alpha.
NCBI Gene Id100462692
Protein NameInterleukin 23, alpha subunit p19
Uniprot IDD6CIQ4
Protein Accession #NP_001172051
Nucleotide Accession #NM_001185122