Clinisciences > IL8 (CXCL8) mouse monoclonal antibody, clone 257, Azide Free
IL8 (CXCL8) mouse monoclonal antibody, clone 257, Azide Free
Cat# AM09178AF-N
Size : 500ug
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IL8 (CXCL8) Mouse Monoclonal Antibody [Clone ID: 257]
| Product Data | |
| Clone Name | 257 |
|---|---|
| Application | ELISA, FN, WB |
| Recommended Dilution | ELISA: React with Human IL-8. Neutralizing: In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell using 50 µg/ml of MAb and 10 ng/ml of human IL-8 in assay. This MAb inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS. Western Blot: Concentration of 0.02-0.1 µg/ml of MAb will allow visualization of 100 ng/lane of human IL-8. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant human IL-8. |
| Specificity | This monoclonal IL8 antibody recognizes both recombinant and native Human Interleukin-8. It does not cross react with Human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted) |
| Buffer | 0.01M PBS, pH 7.0 without preservatives. State: Azide Free State: Lyophilized purified Ig fraction. |
| Reconstitution Method | Restore with Double distillated water to adjust the final concentration to 1.00 mg/ml. |
| Purification | Affinity Chromatography on Protein G. |
| Conjugation | Unconjugated |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | C-X-C motif chemokine ligand 8 |
| Database Link | |
| Background | Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation. |
| Synonyms | CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1 |
| Reference Data | |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Protein Pathways | Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.