Insulin ELISA Kit (Monkey)

Cat# OKCA00217

Size : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKCA00217
Product Info
Predicted Species ReactivityMonkey
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology Insulin ELISA Kit (Monkey) (OKCA00217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Monkey monoclonal antibody specific for Monkey Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Monkey Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Monkey Insulin captured in well.
ELISA Kit Range8 uIU/mL - 140 uIU/mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (μIU/mL)33.65932.92930.34930.0732.01329.228
SD0.0230.0250.0250.0310.0330.029
CV%6.3547.0187.6519.4719.3469.187
ELISA Kit Component
ComponentAmount
Anti-Insulin Microplate96 Well (12 x 8 Well Strips)
Insulin Standard5
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
Sensitivity5 uIU/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of monkey
INS. No significant cross-reactivity or interference between monkey INS and
analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolInsulin

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