L-Lactate Assay Kit, BioAssay™, Fluorometric

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.||Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. This Lactate Assay Kit is based on lactate dehydrogenase-catalyzed oxidation of L-Lactate, in which the formed NADH reduces a probe into a highly fluorescent product. the fluorescence intensity of this product (measured using excitation wavelength and emission wavelength of 530nm and 585 nm, respectively), is proportional to the lactate concentration in the sample.||Applications:|Direct Assays: Lactate in serum, plasma, urine, cell media samples and other biological samples.||Key Features:|1. Sensitive and accurate: Detection limit of 1uM and linearity up to 50uM L-Lactate in 96-well plate assay.||2. Convenient: The procedure involves adding a single working reagent, and reading the fluorescence after 60 minutes. Room temperature assay.| |3. High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.||Kit Components: |L1011-04B1: Assay Buffer, 1x10ml |L1011-04B2: NAD Solution, 1x1ml|L1011-04B3: Enzyme A, 1x120ul |L1011-04B4: Enzyme B, 1x120ul |L1011-04B5: Probe, 1x750ul|L1011-0B6: Standard, 1x1ml ||Storage and Stability:|Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.