Lactate Dehydrogenase B (LDHB) BioAssay™ ELISA Kit (Human)

The Human Lactate Dehydrogenase B (LDHB) ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LDHB in human serum, plasma, tissue homogenates and other biological fluids.||Detection Range:|0.312-20ng/ml||Sensitivity:|0.133ng/ml||Intra-Assay CV:|<10%||Inter-Assay CV:|<12%||Kit Components:|*026420A: Microtiter Plate, 1x48wells or 1x96 wells, Pre-coated; ready to use.|*026420B: Standard, 2x1vial |026420C: Standard Diluent, 1x10ml or 1x20ml|*026420D: Detection Reagent A (green), 1x60ul or 1x120ul |*026420E: Detection Reagent B (red), 1x60ul or 1x120ul |026420F: Assay Diluent A, 1x6ml or 1x12ml|026420G: Assay Diluent B, 1x6ml or 1x12ml|026420H: TMB Substrate, 1x4.5ml or 1x9ml|026420K: Stop Solution, 1x3ml or 1x6ml|026420L: Wash Buffer, 30X, 1x10ml or 1x20ml ||Precaution:|The Stop Solution (026420K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.||Storage and Stability:|Store *026420A, *026420B, *026420D and *026420E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.|3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediately.