Laminin gamma 1,2,3 (LAMB 2, LAMB2, LAMC 1, LAMC1, Laminin B2 Chain, Laminin gamma 1, Laminin gamma 1 Chain, B2T, BM600, Cell-scattering Factor 140kD Subunit, CSF 140kD Subunit, CSF, EBR2, EBR2A, Epiligrin Subunit gamma, Kalinin Subunit gamma, Kalinin/Nicein/Epiligrin 100kD Subunit, Ladsin 140kD Subunit, LAMB2T, LAMC2, LAMC2_HUMAN, Laminin 5 gamma 2 Subunit, Laminin B2t Chain, Laminin gamma 2, Laminin gamma 2 Chain, Laminin Subunit gamma-2, Laminin-5 Subunit gamma, LAMNB2, Large Adhesive Scatter Factor 140kD Subunit, Nicein Subunit gamma, LAMA 1, LAMA, LAMA1, LAMB1, Lamc3, LAMC3_HUMAN, Laminin A Chain, Laminin alpha 1, Laminin alpha 1 Chain, Laminin B1 Chain, Laminin Subunit beta 1, Laminin Subunit gamma-3, Laminin-12 Subunit gamma, Laminin-14 Subunit gamma, Laminin-15 Subunit gamma)

Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an alpha-chain, a beta-chain, and a gamma-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.||Applications: |Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.|Immunohistochemistry (FFPE): 0.5-1ug/ml, Requires HIER using sodium citrate buffer pH6.0|Immunocytochemistry: 0.5-1ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.