LAMP1 antibody [GT25212]
Cat# GTX634336-25ul
Size : 25ul
Marca : Genetex
Host | Mouse |
---|---|
Clonality | Monoclonal |
Clone Name | GT25212 |
Isotype | IgG2b |
Application | WB, ICC/IF, IHC-Fr |
Reactivity | Human |
Package | 100 μl, 25 μl |
Summary
APPLICATION
Application Note
Application | Recommended Dilution |
---|---|
WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-Fr | Assay dependent |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX634336 ICC/IF ImageLAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:2000.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.Blue: Hoechst 33342 staining. |
GTX634336 WB ImageWild-type (WT) and LAMP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
GTX634336 WB Image HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract) |
GTX634336 WB ImageHeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
Application Reference
REVIEW
SDS | |
---|---|
PBS.pdf |