LOC100507703 Mouse Monoclonal Antibody [Clone ID: BB7.2]
Cat# SM1818A
Size : 500ug
Contatta il distributore locale :
| Product Data | |
| Clone Name | BB7.2 |
|---|---|
| Application | FC, FN, IHC, IP |
| Application | Flow Cytometry: Use 10 µl of 10 µg/ml of diluted antibody to label 106 cells or 100 µl whole blood. Immunoprecipitation. Functional Assays: 5-10 µg/ml (Inhibition of MHC resticted cytotoxicity). Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil tissue. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Papain solubilised HLA-A2. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
| Specificity | This antibody recognizes the Human HLA-A2 histocompatibility antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. BB7.2 may be used for the Flow cytometric detection of HLA-A2 expression and has also been used for Immunoaffinity purification of HLA-A2 molecules. Functionally the antibody is reported to inhibit MHC restricted cellular cytotoxicity. |
| Buffer | PBS without stabilizer or preservative State: Low Endotoxin State: Liquid purified IgG fraction |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A from tissue culture supernatant. |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Database Link | |
| Background | Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D). |
| Synonyms | HLAA2, MHC class I antigen A-2 |
| Reference Data | |
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