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> Macrophages (Monocytes) (APC)

Macrophages (Monocytes) (APC)

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Cat# 227445-100T

Size : 100Tests

Marca : US Biological

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227445 Macrophages (Monocytes) (APC)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P06702, P05109
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
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Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain.The antigen recognized is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: Neat-1:5, 10ul labels 10e6 cells in 100ul|Optimal dilutions to be determined by the researcher.||Hybridoma: |NS-1 myeloma cells with spleen cells from Balb/c mice||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2617 (MAC387)|Host: mouse|Source: human|Concentration: Not Determined |Form: Supplied as a lyophilized powder in PBS, 1% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC). Reconstitute with 1ml ddH2O. |Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Human monoocytes.|Specificity: Recognizes human Macrophages. Species Crossreactivity: equine, porcine, canine, rabbit, cynomolgus and rhesus monkey, bovine, guinea pig, rat, feline, goat, baboon, fallow deer, pygmy hippopotamus,mink, marmoset.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human monoocytes.
Form
Supplied as a lyophilized powder in PBS, 1% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC). Reconstitute with 1ml ddH2O.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human Macrophages. Species Crossreactivity: equine, porcine, canine, rabbit, cynomolgus and rhesus monkey, bovine, guinea pig, rat, feline, goat, baboon, fallow deer, pygmy hippopotamus,mink, marmoset.
References
1. Brandtzaeg, P. et al. (1988) MAC387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J. Clin. Path. 41: 963-970. 2. Brandtzaeg, P. et al. (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology. 21: 191-196. 3. Flavell, D.J. et al . (1987) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35: 1217-1226. 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques Vet. Immunol. Immunopathol. 71: 321-334. 5. Ramsay, A. D. et al. (1991) Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies. J. Pathol. 164: 321-328. 6. Christgau, M. et al. (1998) Characterization of Immunocompetent cells in the diseased canine periodontium. J. Histochem. Cytochem. 46: 1443-1454. 7. Perez, J. et al. (1999) Immunohistochemical study of the inflammatory infiltrate associated with equine squamous cell carcinoma J. Comp. Path. 121: 385-397. 8. Obert, L. et al. (2002) Early pathogenesis of transmucosal Feline Immunodeficiency Virus infection. J. Virol. 76: 6311-6322. 9. Malik, N. et al. (1998) Apoptosis and Cell proliferation after porcine coronary angioplasty. Circulation. 98: 1657-1665. 10. Bagavant, H. et al. (2002) Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)). Am. J. Pathol. 160: 141-149. 11. Sanchez, J. et al. (2011) Microscopical and Immunological Features of Tuberculoid Granulomata and Cavitary Pulmonary Tuberculosis in Naturally Infected Goats. J Comp Pathol. Feb 17. [Epub ahead of print] 12. Pomeroy, I.M. et al. (2005) Demyelinated neocortical lesions in marmoset autoimmune encephalomyelitis mimic those in multiple sclerosis. Brain. 128: 2713-21. 13. Vranckx, K. et al. (2012) Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain BMC Veterinary Research 8:24. 14. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation J Clin Pathol 3 sept [epub ahead of print] 15. García­Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama). Vet Immunol Immunopathol. 149: 66-75. 16. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells. Cell Medicine. 4: 1-11. 17. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae. PLoS Negl Trop Dis. 2013 Mar; 7(3): e2138. 18. Tellez, A. et al. (2014) Experimental evaluation of efficacy and healing response of everolimus-eluting stents in the familial hypercholesterolemic swine model: a comparative study of bioabsorbable versus durable polymer stent platforms Coron Artery Dis. Mar 17. [Epub ahead of print]