Application
| WB, ICC |
|---|---|
| Primary Accession | Q5PSV9 |
| Other Accession | NP_001010833.2 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human, Mouse, Chimpanzee, Bovine |
| Clonality | Monoclonal |
| Description | Mouse Anti-Mouse MDC1 Monoclonal IgG1 |
| Target/Specificity | Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus. |
| Other Names | Nuclear factor with BRCT domains1 Antibody, mediator of DNA damage checkpoint 1 Antibody |
| Clone Names | P2B11 |
| Immunogen | GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis. |
| Cellular Localization | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.
References
1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362.
2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199.
3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814.
4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.