Mouse bACE1 (Beta-Site APP Cleaving Enzyme 1) Sandwich ELISA Kit Ready-To-Use

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	Bace1
Gene ID:	23821
Uniprot ID:	BACE1_MOUSE
Immunogen Region:	Ready-To-Use
Sample Type:	serum, plasma, tissue homogenates, cerebrospinal fluid or other biological fluids.

Tissue Specificity	Expressed in the brain, specifically in neurons and astrocytes (at protein level).
Post Translational Modifications	N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions. Palmitoylation mediates lipid raft localization. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface.
Function	Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1.
Protein Name	Beta-Secretase 1 Aspartyl Protease 2 Asp2 Asp 2 Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 Beta-Site App Cleaving Enzyme 1 Memapsin-2 Membrane-Associated Aspartic Protease 2
Database Links
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Golgi Apparatus Trans-Golgi Network Endoplasmic Reticulum Endosome Late Endosome Early Endosome Cell Surface Cytoplasmic Vesicle Membrane Membrane Raft Lysosome Recycling Endosome Cell Projection Axon Dendrite Predominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi Compartments A Small Portion Is Also Found In The Endoplasmic Reticulum Endosomes And On The Cell Surface Colocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Wich Blocks Targeting To Late Endosomes And Lysosomes Retrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner
Alternative ELISA Names	Beta-Secretase 1 ELISA kit Aspartyl Protease 2 ELISA kit Asp2 ELISA kit Asp 2 ELISA kit Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 ELISA kit Beta-Site App Cleaving Enzyme 1 ELISA kit Memapsin-2 ELISA kit Membrane-Associated Aspartic Protease 2 ELISA kit Bace1 ELISA kit Bace ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance