Mouse Ki67P (Ki-67 Protein) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Mki67
Gene ID:	17345
Uniprot ID:	KI67_MOUSE
Sample Type:	tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Tissue Specificity	Mainly present in proliferating cells (at protein level).
Post Translational Modifications	Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Function	Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Protein Name	Proliferation Marker Protein Ki-67 Antigen Identified By Monoclonal Antibody Ki-67 Homolog Antigen Ki-67 Homolog Antigen Ki67 Homolog
Database Links
Cellular Localisation	Chromosome Nucleus Nucleolus Associates With The Surface Of The Mitotic Chromosome The Perichromosomal Layer And Covers A Substantial Fraction Of The Mitotic Chromosome Surface Associates With Satellite Dna In G1 Phase Binds Tightly To Chromatin In Interphase Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes Predominantly Localized In The G1 Phase In The Perinucleolar Region In The Later Phases It Is Also Detected Throughout The Nuclear Interior Being Predominantly Localized In The Nuclear Matrix
Alternative ELISA Names	Proliferation Marker Protein Ki-67 ELISA kit Antigen Identified By Monoclonal Antibody Ki-67 Homolog ELISA kit Antigen Ki-67 Homolog ELISA kit Antigen Ki67 Homolog ELISA kit Mki67 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance